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KMID : 0606920150230050400
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Biomolecules & Therapeutics
2015 Volume.23 No. 5 p.400 ~ p.406
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Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid
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Peng Jin Fu
Liu Li
Guo Cheng Xian
Liu Shi Kun
Chen Xiao Ping
Huang Li Hua
Xiang Hong
Huang Zhi Jun
Yuan Hong
Yang Guo Ping
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Abstract
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MicroRNAs (miRNAs) are a family of non-coding RNA that are able to adjust the expression of many proteins, including ATP-binding cassette transporter and organic cation transporter. We sought to evaluate the effect of miR-511 on the regulation of OATP1B1 expression by free fatty acids. When using free fatty acids to stimulate Chang liver cells, we found that the expression of miR-511 increased significantly while the expression of OATP1B1 decreased. We also proved that SLCO1B1 is the target gene of miR-511 with a bioinformatics analysis and using the dual luciferase reporter assay. Furthermore, the expressions of SLCO1B1 and OATP1B1 decreased if transfecting Chang liver cells with miR-511, but did not increase when transfecting the inhibitors of miR-511 into steatosis cells. Our study indicates that miR-511 may play an important role in the regulation of OATP1B1 expression by free fatty acids.
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KEYWORD
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miR-511, OATP1B1, SCLO1B1, Expression, Free fatty acid
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